On Friday 11 November 2005 10:37 am, Jake Michaelson wrote:
I'll clarify a little and hopefully this will make more sense (thanks for the friendly encouragement):
Let's say I have 6 samples and am looking at 3 genes, with intensities in a matrix as follows:
> genes=cbind(ABC1=c(3,4,4,5,6,3), ABC2=c(4,3,4,7,7,8), ABC3=c(8,7,8,6,3,2))
> genes
ABC1 ABC2 ABC3
[1,] 3 4 8 [2,] 4 3 7 [3,] 4 4 8 [4,] 5 7 6 [5,] 6 7 3 [6,] 3 8 2
###plot the image
>image(1:nrow(genes), 1:ncol(genes), genes, axes = FALSE, xlab = "", ylab =
"", col=cm.colors(256))
###label the axes
>axis(1, 1:nrow(genes), labels = rownames(genes), las = 2, line = -0.5, tick =
0)
>axis(2, 1:ncol(genes), labels = colnames(genes), las = 2, line = -0.5, tick =
0)
Now let's say (I'm just making these numbers and the scenario up here -- for simplicity's sake) that I had run a statistical analysis previously and wanted to label the genes that showed a significance of p< 0.05. Let's say that ABC1 and ABC3 had p<0.05 (assume that these values would be in a two-column matrix with the gene name and its p-value).
> sig=cbind(name=c("ABC1", "ABC2", "ABC3"), pvalue=c(0.005, 0.1, 0.001))
> sig
name pvalue
[1,] "ABC1" "0.005" [2,] "ABC2" "0.1" [3,] "ABC3" "0.001"
I now want these (the names of the significant genes) to be labeled in red rather than black on the plot. I would eventually write a script that would generate a large number of these images, each with a different set of genes. I would like to insert some sort of conditional formatting so that if that gene meets the significance threshold, the name is automatically plotted in red on the plot.
I hope this is more clear and effective in explaining what I'm looking for.
Thanks,
--Jake
> Hi all,
>
> I am interested in plotting a heatmap of a set of genes. I would like the
> text labels of these genes to be colored red rather than black if they meet
> certain statistical criteria (using an if statement). I'm not sure how to
> change individual color labels without changing them all. Can anyone
> provide some insight on how to do this?
>
> Thanks in advance,
>
> Jake
>
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