# Re: [R] ANOVA 1 too few degrees of freedom

From: S Ellison <S.Ellison_at_lgc.co.uk>
Date: Thu, 05 May 2011 19:55:03 +0100

>>> Rovinpiper <david.j.meehan_at_gmail.com> 04/05/2011 22:43 >>>
>So this seems to indicate that I have what I want. I have two
>respiration data points at each plot on each day.

Yes; if you had only Plot+Day you'd have a completely balanced full factorial ... for Plot and Day.

```                                                              Df  Sum
Sq    Mean Sq    F value           Pr(>F)

Combined.Trt                                           1   52.80
52.805     2.0186e+30  < 2.2e-16 ***
as.factor(Combined.Plot)                         10  677.69    67.769
2.5907e+30  < 2.2e-16 ***
as.factor(Combined.Day)                         16  2817.47  176.092
6.7317e+30  < 2.2e-16 ***
Combined.Trt:as.factor(Combined.Day)   16   47.82      2.989
```
1.1426e+29 < 2.2e-16 ***
as.factor(Combined.Day):Combined.Plot 160 611.21 3.820 1.4604e+29 < 2.2e-16 ***
```Residuals                                                 204    0.00
```
0.000

Now, your Day:Plot combination has 17*12=204 combinations. And you are showing 204 residual DOF, which is exactly right for two observations per group and 204 groups. That's exactly right for the model Day+Plot+Day:Plot.

But you have Trt in the model as well. Where are the Treatment DoF coming from?
Clearly, the treatment DoF has not come out of the residual term. That means the treatment DoF must have come from somewhere else. Since you've lost 2 instead of 1 DoF from Plot and kept your n[Day]-1 degrees of freedom for Day, I would guess that Plot is nested in Trt. If that is the case, you'd 'obviously' expect n[Plot]-2 dof for Plot. And indeed i could replicate your ANOVA table DoF with

Trt<-gl(2,204)
Plot<-gl(12,34) #Note that Plot is NOT fully crossed with Trt; each Trt has only 6 Plots
Day <- gl(17,2,408)
y<-rnorm(408)
anova(lm(y~Trt+Plot+Day+Trt:Day+Day:Plot))

So that would be one data structure that would explain your DoF.

But if that is indeed the structure, there are other concerns that you may need to look out for.
First, compare the two models
anova(lm(y~Trt+Plot+Day+Trt:Day+Day:Plot)) #and
anova(lm(y~Trt+Plot+Day+Day:Plot+Trt:Day))

Same terms in the model specification, but different DoF and Trt:Day has completely vanished from the second ANOVA table. Something is clearly either aliased, unbalanced or incorrectly specified. In my presumed version of events, because the Plot is nested in Trt, Day:Plot is also nested in Trt. To get _consistent_ results independent of model order you would need to reflect that additional nesting in the model:

anova(lm(y~Trt+Plot+Day+Trt:Day:Plot+Trt:Day)) #vs
anova(lm(y~Trt+Plot+Day+Trt:Day+Trt:Day:Plot))

and this time, the two models give identical results for all rows in the table. Somewhat reassuringly
anova(lm(y~Day+Trt/Plot+Trt:Day+Trt:Day:Plot)) and even more reassuringly car's Anova does too.

But there's another more fundamental thing that may suggest this is still not quite the right thing to do. If Plot is nested in Trt, it matters whether plot is random or fixed when asking about the treatment effect. I'd guess Plot is random. If plot is random and nested in Trt it would not be appropriate to simply compare any calculated Trt MS with the residual MS. Rather, one would compare the Trt MS with the Trt:Plot interaction. Or perhaps resort to a mixed effects model using lme (if Plot is the only random term) or lmer if Plot and Day are both random.

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