[R] Repost: Estimation when interaction is present: How do I get get the parameters from nlme?

From: John S. Walker <jsw9c_at_uic.edu>
Date: Fri 16 Jun 2006 - 01:36:39 EST


Gday,

This is a repost since I only had one direct reply and I remain mystified- This
may be stupidity on my part but it may not be so simple.

In brief, my problem is I'm not sure how to extract parameter values/effect sizes from a nonlinear
regression model with a significant interaction term.

My data sets are dose response curves (force and dose) for muscle that also have two treatments applied
Treatment A (A- or A+) and Treatment B (B-/B+). A single muscle was used for each experiment - a full dose response curve and one treatment from the matrix A*B (A-/B-, A+/B-, A-/B+ and A+,B+). There are 8 replicates for each combination of treatments We fit a dose response curve to each experiment with parameters upper, ed50 and slope; we expect treatment A to change upper and ed50. We want to know if treatment B blocks the effect of treatment A and if so to what degree.
This is similar to the Ludbrook example in Venables and Ripley, however they only had one treatment and I have two.

my approach

The dataframe is structured like this:

expt 	treatA 	treatB 	dose 	force.
1	-		-		0.1		20
1	-		-		0.2		40
...
4	+		+		0.1		20
4	+

I used a groupedData object: mydata=groupedData(force ~ dose | expt)

I used an nlme obect to model the data as follows (pseudocode):

myfit.nlme <- nlme(force ~ ss_tpl(dose, upper, ed50,slope), fixed=list(ed50~factor(treatA)*factor(treatB)))

The function ss_tpl is a properly debugged and fully functional selfstarting three parameter logistic function that I wrote- no problem here. In my analysis
I also included fixed terms for the other fit parameters; upper and slope, but my main problem is with the ed50 so that's all I've included here.

Running an anova on the resulting object (anova(myfit.nlme) I found the A -/B- (control) to
be significantly different from zero, treatment A was significantly different, treatment B had no significant effect and there was a significant interaction between treatment A and treatment B.

The interaction term is likely to be real. The treatments are on sequential steps in a pathway and treatment B may be blocking the effect of treatment A, i.e. treatment B alone has no effect because it blocks a pathway that is not active, treatment A reduces force via this pathway and treament B therefore blocks the effect of treatment A when used together.

 From what I understand, please correct me if I'm wrong, the parameter estimates from summary(model.nlme) are not correct for main effects if a significant interaction is present. For example in my data treatment B alone has no signifcant effect in the anova but the interaction term A:B is significant. I believe The summary estimate for B is the estimate across all levels of A. What I want to do is pull out the estimate for B when A is not present. I suppose I can do it manually from the list of coefficients from nls or fit a oneway model with treatment levels A, B, AB. But I was kind of hoping there was some extractor function.

The reason I need this is that the co-authors want to include a table of parameter values with std errs or confidence intervals ala:

Treat	upper	ed50	slope
A-/B-		x		x		x		<- shows value for comparison to control studies
A+/B-	x		x		x		<-Shows A is working0
A-/B+	x		x		x		<- Shows B has no effect alone
A+/B	+	x		x		x 		<-shows B blocks A (not necessarily total)
	

So back to my question,How do I extract estimates of the parameters from my model object for a
specific combination of factors including the interaction term.

   i.e. what is the ed50 (and std err) for A-/B-, A+/B-, A-/B+, A+/B+ ?

I think this is a fair question and one that many biomedical scientists would need.



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